One-pot analysis of enantiomeric excess of free amino acids by electrospray ionization mass spectrometry

Takashi Nakakoji; Hirofumi Sato; Daisuke Ono; Hiroyuki Miyake; Eiko Mieda; Satoshi Shinoda; Hiroshi Tsukube; Hideya Kawasaki; Ryuichi Arakawa; Motohiro Shizuma

doi:10.1039/d1ra06542d

One-pot analysis of enantiomeric excess of free amino acids by electrospray ionization mass spectrometry

RSC Adv. 2021 Nov 10;11(57):36237-36241. doi: 10.1039/d1ra06542d. eCollection 2021 Nov 4.

Affiliations

¹ Department of Chemistry, Graduate School of Science, Osaka City University Sugimoto, Sumiyoshi-ku Osaka 558-8585 Japan miyake@sci.osaka-cu.ac.jp.
² Osaka Research Institute of Industrial Science and Technology Morinomiya, Joto-ku Osaka 536-8553 Japan shizuma@omtri.or.jp.
³ Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University Yamate-cho, Suita Osaka 564-8680 Japan.

Abstract

An electrospray ionization mass spectrometric method for the simultaneous analysis of the enantiomeric excess of free amino acids, without chromatographic separation, was demonstrated using a quasi-racemic mixture of deuterium-labelled and unlabelled chiral copper(ii) complexes. This convenient method enables the simultaneous high-sensitivity determination of the enantiomeric excess of 12 amino acids.