Isolation and biological activities of compounds from Rumex vesicarius L. and their use as a component of a synbiotic preparation

Ahmed Elbermawi; Mohamed Samir Darwish; Asmaa A El-Awady; Ahmed A Zaki; Longxin Qiu; Reham M Samra

doi:10.1016/j.fochx.2022.100306

Isolation and biological activities of compounds from Rumex vesicarius L. and their use as a component of a synbiotic preparation

Food Chem X. 2022 Apr 9:14:100306. doi: 10.1016/j.fochx.2022.100306. eCollection 2022 Jun 30.

Authors

Ahmed Elbermawi¹, Mohamed Samir Darwish², Asmaa A El-Awady², Ahmed A Zaki^{1

3}, Longxin Qiu^{4

5}, Reham M Samra¹

Affiliations

¹ Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, 35516 Egypt.
² Dairy Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
³ Department of Pharmacognosy, Faculty of Pharmacy, Horus University-Egypt, New Damietta 34518, Egypt.
⁴ Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan University, Longyan, 364012, P.R. China.
⁵ Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, P.R. China.

Abstract

The study evaluated prebiotic potential and the enzyme inhibition of extracts and isolated compounds of Rumex vesicarius (ruby dock), family Polygonaceae. Eight known compounds were identified in the roots of R. vesicarius. Extracts and compounds (1-8) increased the growth rate of Escherichia coli Nissle 1917 differentially compared to controls. The highest prebiotic index (PI) and activity score was recorded for EcN in the presence of compound 4, followed by, in descending order, petroleum ether, ethyl acetate, and total methanol extracts. The compounds and extracts reduced protease, α-amylase, and angiotensin-converting enzyme activities. This inhibitory activity was positively correlated with PI, P_score, µ_u, and Y_max. These findings suggest that R. vesicarius is a good source of potential prebiotic and can boost beneficial bacteria. It may also be considered promising for treatment of diabetes mellitus, controlling weight, and regulating blood pressure.

Keywords: ACE, Angiotensin-converting enzyme; Angiotensin-converting enzyme; CFU, Colony forming units; EcN, Escherichia coliNissle 1917; Escherichia coli Nissle 1917; Lag, Lag time; NB, Nutrient broth; PI, Prebiotic index; Prebiotic; Protease; Pscore, Prebiotic score; Ruby dock; Td, Doubling time; Ymax, Maximum growth at the stationary phase; µmax, specific growth rate; α- Amylase.