Impacts of molybdate and ferric chloride on biohythane production through two-stage anaerobic digestion of sulfate-rich hydrolyzed tofu processing residue

Abstract

Biohythane production through one-stage anaerobic digestion of sulfate-rich hydrolyzed tofu processing residue has been hampered by high H₂S production. Herein, two-stage anaerobic digestion was investigated with the addition of molybdate (MoO₄^2-; 0.24-3.63 g/L) and ferric chloride (FeCl₃; 0.025-5.4 g/L) to the dark fermentation stage (DF) to improve biohythane production. DF supplemented with 1.21 g/L MoO₄^2- increased hydrogen yield by 14.6% over the control (68.39 ml/g-VS_fed), while FeCl₃ had no effect. Furthermore, the maximum methane yields of methanogenic fermentation were 524.8 and 521.6 ml/g-VS_fed with 3.63 g/L MoO₄^2- and 0.6 g/L FeCl₃ compared to 466.07 ml/g-VS_fed of the control. The maximum yields of biohythane and energy were 796.7 ml/g-VS_fed and 21.8 MJ/kg-VS_fed with 0.6 g/L FeCl₃ when the sulfate removal efficiency was 66.7%, and H₂S content was limited at 0.08%. Therefore, adding 0.6 g/L FeCl₃ is the most beneficial in improving energy recovery and sulfate removal with low H₂S content.

Keywords: Dark fermentation; Hydrogen sulfide; Methanogenic fermentation; Pretreatment; Sulfate reduction.