Cholecalciferol supplementation lowers leptin and TMAO but increases NO and VEGF-A levels in obese vitamin D deficient patients: Is it one of the potential cardioprotective mechanisms of vitamin D?

Mateusz Ozorowski; Michał Wiciński; Łukasz Wróbel; Anna Fajkiel-Madajczyk

doi:10.1186/s12986-022-00666-4

Cholecalciferol supplementation lowers leptin and TMAO but increases NO and VEGF-A levels in obese vitamin D deficient patients: Is it one of the potential cardioprotective mechanisms of vitamin D?

Nutr Metab (Lond). 2022 Apr 29;19(1):31. doi: 10.1186/s12986-022-00666-4.

Authors

Mateusz Ozorowski¹, Michał Wiciński², Łukasz Wróbel², Anna Fajkiel-Madajczyk²

Affiliations

¹ Department of Pharmacology and Therapeutics, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, M. Curie 9, 85-090, Bydgoszcz, Poland. mateusz-ozorowski@o2.pl.
² Department of Pharmacology and Therapeutics, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, M. Curie 9, 85-090, Bydgoszcz, Poland.

Abstract

Background: Vitamin D deficiency is one of the most common health issues in developed countries. Obese patients are most at risk of having serum 25-hydroxyvitamin D₃ (25(OH)D₃) levels that are too low due to the accumulation of vitamin D in adipose tissue. While the effects of a deficiency on the skeletal or immune system are known, the effects on the cardiovascular system are not yet clear. Our study investigates the effect of cholecalciferol supplementation in obese patients on selected biomarkers associated with cardiovascular diseases (CVDs).

Methods: The study enrolled 33 obese patients with insufficient 25(OH)D₃ levels. For three months, the subjects supplemented with cholecalciferol at a dose of 2000 IU/day. Concentrations of nitric oxide (NO), vascular endothelial growth factor A (VEGF-A), leptin, trimethylamine N-oxide (TMAO) and soluble suppression of tumorigenicity 2 (sST2) were measured in baseline samples using ELISA (BioTek EPOCH). 25(OH)D₃ levels measured on Beckman Coulter DXI 800 by chemiluminescence method.

Results: After supplementation, 25(OH)D₃ levels increased significantly. Normal levels were achieved in most patients. A statistically significant reduction leptin and TMAO levels was observed. At the same time, NO and VEGF-A levels increased statistically significantly.

Conclusion: This study indicates that restoring normal 25(OH)D₃ levels in obese people reduces the concentration of pro-inflammatory factors associated with cardiovascular diseases. Reducing inflammation and the potential impact on vascular reactivity leads to the conclusion that cholecalciferol supplementation in obese patients may benefit the cardiovascular system.

Keywords: Cardiovascular diseases; Inflammation; Leptin; Markers; NO; Obesity; TMAO; VEGF-A; Vitamin D; sST2.