A comparative study of endogenous phospholipase A2 inhibitors in the serum of Brazilian pit vipers

Victor Koiti Kavazoi; Caroline Serino-Silva; Caroline Fabri Bittencourt Rodrigues; Daniela Miki Hatakeyama; Daniel Carvalho de Souza; Kathleen Fernandes Grego; Karen de Morais-Zani; Anita Mitico Tanaka-Azevedo

doi:10.1016/j.toxicon.2022.04.011

A comparative study of endogenous phospholipase A₂ inhibitors in the serum of Brazilian pit vipers

Toxicon. 2022 Jul 15:213:87-91. doi: 10.1016/j.toxicon.2022.04.011. Epub 2022 Apr 27.

Authors

Victor Koiti Kavazoi¹, Caroline Serino-Silva¹, Caroline Fabri Bittencourt Rodrigues¹, Daniela Miki Hatakeyama¹, Daniel Carvalho de Souza², Kathleen Fernandes Grego², Karen de Morais-Zani¹, Anita Mitico Tanaka-Azevedo³

Affiliations

¹ Laboratório de Herpetologia, Instituto Butantan, São Paulo, SP, Brazil; Interunidades Em Biotecnologia, Universidade de São Paulo (USP) - Instituto Butantan (IBu) - Instituto de Pesquisas Tecnológicas (IPT), São Paulo, SP, Brazil.
² Laboratório de Herpetologia, Instituto Butantan, São Paulo, SP, Brazil.
³ Laboratório de Herpetologia, Instituto Butantan, São Paulo, SP, Brazil; Interunidades Em Biotecnologia, Universidade de São Paulo (USP) - Instituto Butantan (IBu) - Instituto de Pesquisas Tecnológicas (IPT), São Paulo, SP, Brazil. Electronic address: amt.azevedo@uol.com.br.

PMID: 35487313
DOI: 10.1016/j.toxicon.2022.04.011

Abstract

This work compared the presence of phospholipase A₂ inhibitors (PLIs) in the serum of 19 snake species maintained at Instituto Butantan to better understand the mechanisms of venom resistance in snakes and improve the treatment of snakebite. PLI was isolated from blood of 19 snake species by one-step chromatography and identified in all samples, besides its identity was confirmed through the interaction with both phospholipase A₂ and anti-γPLI. These findings highlight the diversity of snake serum PLIs and emphasize the importance of structure-function studies.

Keywords: Natural resistance; Phospholipase A(2); Phospholipase A(2) inhibitors; Snakebite.

MeSH terms

Animals
Brazil
Crotalinae*
Phospholipase A2 Inhibitors / chemistry
Phospholipases A2
Snakes

Substances

Phospholipase A2 Inhibitors
Phospholipases A2