Mycoplasma hyopneumoniae detection by PCR in naturally infected finishing pigs

Karine Ludwig Takeuti; Taís Regina Michaelsen; Carla Sabedot; Ricardo Yuiti Nagae; Ricardo Augusto Neves Forner; Angela Mazzarollo; David Emilio Santos Neves de Barcellos; Maria Pieters

doi:10.1016/j.mimet.2022.106475

Mycoplasma hyopneumoniae detection by PCR in naturally infected finishing pigs

J Microbiol Methods. 2022 Jun:197:106475. doi: 10.1016/j.mimet.2022.106475. Epub 2022 Apr 26.

Authors

Karine Ludwig Takeuti¹, Taís Regina Michaelsen², Carla Sabedot², Ricardo Yuiti Nagae³, Ricardo Augusto Neves Forner¹, Angela Mazzarollo¹, David Emilio Santos Neves de Barcellos¹, Maria Pieters⁴

Affiliations

¹ Department of Animal Medicine, College of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
² Seara Alimentos, Itajaí, SC, Brazil.
³ Department of Animal Medicine, College of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Seara Alimentos, Itajaí, SC, Brazil.
⁴ Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States; Swine Disease Eradication Center, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States. Electronic address: piet0094@umn.edu.

PMID: 35487292
DOI: 10.1016/j.mimet.2022.106475

Abstract

The aim of this study was to compare the sensitivity of different in vivo and post-mortem samples collected from finishing pigs under field conditions on Mycoplasma hyopneumoniae detection by PCR. Results suggested that tracheobronchial secretions and bronchial swabs conferred the highest sensitivity in vivo and post-mortem, respectively.

Keywords: Bronchial swabs; Diagnostic; Enzootic pneumonia; Pigs; Tracheobronchial secretions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Mycoplasma hyopneumoniae* / genetics
Pneumonia of Swine, Mycoplasmal* / diagnosis
Polymerase Chain Reaction / methods
Swine