Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence

V Pragathi Masamsetti; Patrick P L Tam

doi:10.1007/978-1-0716-2281-0_4

Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence

Methods Mol Biol. 2022:2490:39-45. doi: 10.1007/978-1-0716-2281-0_4.

Authors

V Pragathi Masamsetti^{1

2}, Patrick P L Tam^{3

4}

Affiliations

¹ Embryology Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia. pmasamsetti@cmri.org.au.
² Faculty of Medicine and Health, School of Medical Sciences, University of Sydney, Westmead, NSW, Australia. pmasamsetti@cmri.org.au.
³ Embryology Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia.
⁴ Faculty of Medicine and Health, School of Medical Sciences, University of Sydney, Westmead, NSW, Australia.

PMID: 35486237
DOI: 10.1007/978-1-0716-2281-0_4

Abstract

Mouse embryo studies are pivotal for the understanding of early development. Analysis of the spatial and temporal changes of protein expression during development of a mouse embryo allows us to identify the genetic basis of errors of development in animal disease models. Immunofluorescence is a powerful technique to study the localization and variation in expression pattern of specific proteins in cells, tissues, and organs. Detecting the antigens with their specific antibodies labeled with fluorescent probes allows visualization of proteins at the cellular level. Here, we provide the optimized protocol of immunostaining whole mouse embryos at embryonic stages E7.5 to E11.5.

Keywords: Confocal microscopy; Immunostaining; Mouse embryos; Whole mount.

MeSH terms

Animals
Embryo, Mammalian* / metabolism
Fluorescent Antibody Technique
Fluorescent Dyes* / metabolism
Mice
Staining and Labeling

Substances

Fluorescent Dyes