Molecular cloning, inducible expression and function analysis of Epinephelus coioides Sec6 response to SGIV infection

Pin-Hong Li; Jia-Yang He; Yi-Jie Cai; Yu-Si Wei; Xiang-Long Zhu; Jia-Deng-Hui Yang; Shi-Qi Yang; Sheng Zhou; Qi-Wei Qin; Hong-Yan Sun

doi:10.1016/j.fsi.2022.04.039

Molecular cloning, inducible expression and function analysis of Epinephelus coioides Sec6 response to SGIV infection

Fish Shellfish Immunol. 2022 May:124:462-471. doi: 10.1016/j.fsi.2022.04.039. Epub 2022 Apr 26.

Authors

Affiliations

¹ University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, PR China.
² University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China.
³ University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China; Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266000, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, PR China. Electronic address: qinqw@scau.edu.cn.
⁴ University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, PR China. Electronic address: hongyanlucky@scau.edu.cn.

PMID: 35483595
DOI: 10.1016/j.fsi.2022.04.039

Abstract

Exocyst complex component 3 Sec6 of mammals, one of the components of the exocyst complex, participates in numerous cellular functions, such as promoting cell migration and inhibiting apoptosis. In this study, the Sec6 was obtained from Epinephelus coioides, an economically important cultured fish. The full length of E. coioides Sec6 was 2655 bp including a 245 bp 5' UTR, a 154 bp 3' UTR, and a 2256 bp open reading frame (ORF) encoding 751 amino acids, with a molecular mass of 86.76 kDa and a theoretical pI of 5.57. Sec6 mRNA was detected in all the tissues examined, but the expression level is different in these tissues. Using fluorescence microscopy, Sec6 were distributed in both the nucleus and the cytoplasm. After SGIV infection, the expression of E. coioides Sec6 was significantly up-regulated in both trunk kidney and spleen response to Singapore grouper iridovirus (SGIV), an important pathogens of E. coioides. Sec6 could increase the SGIV-induced cytopathic effects (CPE), the expression of the SGIV genes VP19, LITAF, MCP, ICP18 and MCP, and the viral titers. Besides, E. coioides Sec6 significantly downregulated the promoter of NF-κB and AP-1, and inhibited the SGIV-induced apoptosis. The results demonstrated that E. coioides Sec6 might play important roles in SGIV infection.

Keywords: Apoptosis; Epinephelus coioides; Replication; SGIV; Sec6.

MeSH terms

Animals
Bass* / genetics
Bass* / metabolism
Cloning, Molecular
DNA Virus Infections* / veterinary
Fish Diseases*
Fish Proteins / genetics
Fish Proteins / metabolism
Iridovirus*
Mammals / genetics
Mammals / metabolism
Phylogeny
Ranavirus*

Substances

Fish Proteins