Electrochemiluminescence covalent organic framework coupling with CRISPR/Cas12a-mediated biosensor for pesticide residue detection

Yan Li; Fang Yang; Ruo Yuan; Xia Zhong; Ying Zhuo

doi:10.1016/j.foodchem.2022.133049

Electrochemiluminescence covalent organic framework coupling with CRISPR/Cas12a-mediated biosensor for pesticide residue detection

Food Chem. 2022 Sep 30:389:133049. doi: 10.1016/j.foodchem.2022.133049. Epub 2022 Apr 25.

Authors

Yan Li¹, Fang Yang¹, Ruo Yuan¹, Xia Zhong², Ying Zhuo³

Affiliations

¹ Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
² Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: zhonglyx@swu.edu.cn.
³ Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yingzhuo@swu.edu.cn.

PMID: 35483302
DOI: 10.1016/j.foodchem.2022.133049

Abstract

The trace detection of pesticide residue becomes particularly important since increasing attentions have been attached to food safety. Herein, we developed an electrochemiluminescence (ECL) covalent organic framework (COF) based-biosensor for trace pesticide detection coupling with CRISPR/Cas12a-mediated signal accumulation strategy. Firstly, the target conversion was carried out with an aptamer-assembled magnetic spherical nucleic acids, which can convert acetamiprid to activator DNA, triggering the CRISPR/Cas12a to make quenching probes far away from electrode for signal accumulation. The COF with stable and strong ECL was synthesized by a condensation reaction between the perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA) and melamine (MA), due to the highly ordered arrangement of the PTCDA luminescence units among COF structure and the pore confinement effect. Moreover, the designed assay method was successfully employed to detect the residual level of acetamiprid in real sample and expected to be widely used in pesticide-related food safety.

Keywords: Acetamiprid; CRISPR/Cas12a; ECL biosensor; Food safety; PTCA-COF.

MeSH terms

Biosensing Techniques* / methods
CRISPR-Cas Systems
DNA / genetics
Metal-Organic Frameworks*
Pesticide Residues*

Substances

Metal-Organic Frameworks
Pesticide Residues
DNA