Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

Tetsuya Hirata; Takahiro Takekiyo; Yukihiro Yoshimura; Yuko Tokoro; Takeru Ishizaki; Yasuhiko Kizuka; Kosuke Kuroda

doi:10.1039/d2ra01575g

Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

RSC Adv. 2022 Apr 14;12(19):11628-11631. doi: 10.1039/d2ra01575g. eCollection 2022 Apr 13.

Authors

Tetsuya Hirata¹, Takahiro Takekiyo², Yukihiro Yoshimura², Yuko Tokoro¹, Takeru Ishizaki³, Yasuhiko Kizuka¹, Kosuke Kuroda^{3

4}

Affiliations

¹ Institute for Glyco-core Research (iGCORE), Gifu University Gifu Gifu 501-1193 Japan hirata01@gifu-u.ac.jp.
² Department of Applied Chemistry, National Defense Academy Yokosuka Kanagawa 239-8686 Japan take214@nda.ac.jp muki@nda.ac.jp.
³ Faculty of Biological Science and Technology, Institute of Science and Engineering, Kanazawa University Kakuma-machi Kanazawa 920-1192 Japan kkuroda@staff.kanazawa-u.ac.jp.
⁴ NanoMaterials Research Institute, Kanazawa University Kakuma-machi Kanazawa 920-1192 Japan.

Abstract

We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study highlights the potential utility of synthetic zwitterions as novel cryoprotectants.