A taste of the early steps in BAX activation with FLAMBE

Melanie Gil; Marina R Hanna; Vivian Gama

doi:10.1016/j.crmeth.2022.100190

A taste of the early steps in BAX activation with FLAMBE

Cell Rep Methods. 2022 Mar 15;2(3):100190. doi: 10.1016/j.crmeth.2022.100190. eCollection 2022 Mar 28.

Authors

Melanie Gil^{1

2

3}, Marina R Hanna^{1

2

3}, Vivian Gama^{1

2

3

4}

Affiliations

¹ Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.
² Neuroscience Graduate Program, Vanderbilt University, Nashville, TN, USA.
³ Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA.
⁴ Vanderbilt Center for Stem Cell Biology, Vanderbilt University, Nashville, TN, USA.

Abstract

The activation of BAX through intricate intramolecular changes is critical for apoptosis. In this issue of Cell Reports Methods, Gelles et al. report engineering FLAMBE, an elegant fluorescence polarization ligand assay for monitoring the early activation of monomeric BAX via real-time release of a peptide probe, expanding the repertoire of BAX activation assays to the single-molecule level.

Publication types

Research Support, N.I.H., Extramural
Comment

MeSH terms

Apoptosis* / physiology
Fluorescence Polarization
Ligands
Taste*
bcl-2-Associated X Protein / genetics

Substances

bcl-2-Associated X Protein
Ligands

Abstract

Publication types

MeSH terms

Substances

Grants and funding