Exposure to ambient air pollution and osteoarthritis; an animal study

Abyadul Fitriyah; Denis Andreevich Nikolenko; Walid Kamal Abdelbasset; Marwah Suliman Maashi; Abduladheem Turki Jalil; Ghulam Yasin; Mohammed Mustafa Abdulkadhm; G U Samieva; Holya A Lafta; Azher M Abed; Larissa Souza Amaral; Yasser Fakri Mustafa

doi:10.1016/j.chemosphere.2022.134698

Exposure to ambient air pollution and osteoarthritis; an animal study

Chemosphere. 2022 Aug:301:134698. doi: 10.1016/j.chemosphere.2022.134698. Epub 2022 Apr 23.

Affiliations

¹ Faculty of Animal Science, Universitas Nahdlatul Wathan Mataram, Mataram, Indonesia. Electronic address: abyadulfitriyah@yahoo.com.
² Department of Prosthetic Dentistry, Sechenov First Moscow State Medical University, Moscow, Russia.
³ Department of Health and Rehabilitation Sciences, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al Kharj, Saudi Arabia; Department of Physical Therapy, Kasr Al-Aini Hospital, Cairo University, Giza, Egypt.
⁴ Medical Laboratory Science Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
⁵ Faculty of Biology and Ecology, Yanka Kupala State University of Grodno, 230023, Grodno, Belarus; College of Technical Engineering, The Islamic University, Najaf, Iraq.
⁶ Department of Botany University, Bahauddin Zakariya University, Multan, Pakistan.
⁷ Al-Manara College for Medical Sciences, Misan, Iraq.
⁸ Doctor of Medical Sciences, Department of Pathologic Physiology, Samarkand State Medical Institute, Samarkand, Uzbekistan.
⁹ Al-Nisour University College, Baghdad, Iraq.
¹⁰ Department of Air Conditioning and Refrigeration, Al-Mustaqbal University College, Babylon, Iraq.
¹¹ Department of Bioengineering, University of São Paulo (USP) and Minas Gerais State University (UEMG), Frutal, Minas Gerais, Brazil.
¹² Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul, 41001, Iraq.

PMID: 35472612
DOI: 10.1016/j.chemosphere.2022.134698

Abstract

Exposure to air pollution has been associated with many adverse health effects. However, the evidence on the effects on osteoarthritis (OA) is scarce and the potential mechanism is unclear yet. Therefore, this study assessed the effect of exposure to air pollution (gaseous and particulate matter) and OA based on an animal model. We used four groups of female rats, including i) exposure to PMs and gaseous pollutants, ii) exposure only to gaseous pollutants, iii) exposure only to PMs, and iv) control (unexposed) group. The OA biomarkers, i.e., osteocalcin, cartilage oligomeric protein (COMP), and N-Telopeptides of Type I Collagen (NTX-I) and cytokines were measured in the plasma to detect the effect of exposure to ambient air pollution on OA in this animal model. The forced jogging exercises for 1 h and 5 days per week were used to record the physical activities. The median (interquartile range) concentrations of PM_2.5 and PM₁₀ were 35.9 (15.4) and 47.5 (22.5) μg/m³, respectively. The median (interquartile range (IQR)) of PM_2.5, PM₁₀, CO, NO₂, SO₂ and O₃ in the inlet ambient air were 36.9 (16.9), 51.7 (23.6) μg/m³, 16.1 (12.5) ppm, 413.7 (177.1), 334.2 (218.8) and 208.9 (113.1) ppb, respectively. The osteocalcin was significantly lower in PM as well as PM-gaseous exposure groups compared to control. Moreover, expressions of COMP were increased significantly in the PMs and exposure group compared to the control. For the PMs-gaseous exposure group, the COMP expressions were the highest compared to the control group. Similar results were observed for NTX-I. Exposure to PM and gaseous pollutants significantly increased plasma cytokine levels compared to control. Overall, our study showed a significant effect of exposure to PMs and PMs-gaseous exposure with OA in rats. Moreover, we observed a synergistic effect of mixed gaseous-PMs exposure compared to PMs and gaseous pollutants separately.

Keywords: Air pollution; Animal model; Cartilage oligomeric protein; Osteocalcin.

MeSH terms

Air Pollutants* / analysis
Air Pollution* / analysis
Animals
China
Environmental Exposure / analysis
Environmental Pollutants*
Female
Gases
Osteoarthritis* / chemically induced
Osteocalcin
Particulate Matter / analysis
Particulate Matter / toxicity
Rats

Substances

Air Pollutants
Environmental Pollutants
Gases
Particulate Matter
Osteocalcin