In this study, ethanol-type fermentation pretreatment and adding two types of biochar prepared at 600 °C and 1000 °C (referred to as SS600 and SS1000) were combined to alleviate acid accumulation via strengthening direct interspecies electron transfer (DIET) during anaerobic digestion of food waste. Results demonstrated that ethanol production was about 11 g/L after the ethanol-type fermentation at pH of 4-5 for 4 days, accounting for 8.9% of the influent COD of the subsequent methanogenesis. After the ethanol-type fermentation pretreatment, average methane productions of digesters with SS600 and SS1000 addition increased by 86.3% and 64.9% to 618.1 ± 30.1 and 527.3 ± 25.4 mL/g VS under solid retention time (SRT) of 25 d respectively, and the conductivity of sludge increased by 95.3% and 65.3% compared to digester without biochar addition. Furthermore, adding biochar also could accelerate the recovery of acidification digester. The relative abundance of Methanothrix performing DIET were enriched with SS600. These results suggested that coupling ethanol-type fermentation with biochar addition could strengthen DIET to resist the shocks of high organic loading rate.
Keywords: Acid accumulation; Anaerobic digestion; Biochar; DIET; Ethanol-type fermentation.
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