Methane production from peroxymonosulfate pretreated algae biomass: Insights into microbial mechanisms, microcystin detoxification and heavy metal partitioning behavior

Abstract

This study investigated the methane production potential of algal biomass by anerobic digestion with the addition of peroxymonosulfate (PMS), the removal of microcystin were analyzed and discussed. The microcystin concentration in the collected algal sludge was 1.20 μg/L in the liquid phase and 1393 μg/g in the algal sludge before anaerobic fermentation. The microcystin concentration decreased to 0.20-0.35 μg/L in the liquid phase and 4.16-11.51 μg/g in the sludge phase after 60 days of digestion. The initial PMS dose and residue microcystin concentration could be simulated with a logarithmic decay model (R² > 0.87). Anaerobic digestion could recover energy from algal source in the form of methane gas, which was not affected in the presence of microcystin, and the microcystin removal rate was >99%. Digestion decreased the total contents of Cd and Zn in the liquid phase and increased the total contents of Cr and Pb in the liquid phase. The microbial community and function prediction results indicated that the PMS0.1 system had the highest methane production, which was attributed to the high abundance of Mechanosaeta (40.52%). This study provides insights into microbial mechanisms, microcystin detoxification and the heavy metal partitioning behavior of the algal biomass during methane production.

Keywords: Algae; Anaerobic fermentation; Function prediction; Metabolic pathway; Microbial process.