The development of multi-level anti-counterfeiting techniques is of great significance for economics and security issues, particularly the newly emerged temporal-domain techniques based on lifetime coding. However, the intricate reading methods required to obtain temporal-level information are inevitably cumbersome and expensive, which greatly limits the practical applications of these techniques. Herein, we report a novel, unclonable time-domain anti-counterfeiting strategy for the first time, which is achieved using photo-responsive ZnSe:Mn/ZnS quantum dots (QDs) with dynamic luminescence and can be authenticated by the naked eye. Through introducing electron traps and constructing cascade electron channels in the QDs, the binary temporary photo-response is tailored and manifested as distinctive response rates between the band-edge and Mn 4T1-6A1 transition emissions. Impressively, the generated photo-response is instantaneous, is capable of delayed recovery, and can be visibly detected under UV irradiation. The prospective use of colorless, nontoxic aqueous-phase ZnSe:Mn/ZnS QDs provides a new idea and important guidance for developing the next generation of multi-level anti-counterfeiting techniques without the need for complex time-gated decoding instrumentation.