Streptomyces liliifuscus sp. nov and an anti-ginger plague agent Streptomyces liliiviolaceus sp. nov, two novel species isolated from soil of Lilium lancifolium

Abstract

Two novel strains of actinobacteria, ZYC-3^T and BH-SS-21^T, were isolated from Hunan Province, PR China. The fermentation broth of BH-SS-21^T inhibited the rapid spread of ginger blast, unlike that of ZYC-3^T. The taxonomic characteristics of ZYC-3^T and BH-SS-21^T were defined using a polyphasic approach. The analysis of the full-length 16S rRNA gene sequence revealed that ZYC-3^T and BH-SS-21^T represented members of the genus Streptomyces. ZYC-3^T had less than 98.7% sequence similarities to all species of the genus Streptomyces, while BH-SS-21^T exhibited 99.97, 98.95, 98.83, 98.82, 98.75 and less than 98.7% sequence similarities to 'Streptomyces dioscori' A217, Streptomyces ederensis JCM 4958^T, Streptomyces glomeroaurantiacus NBRC 15418^T, Streptomyces aurantiacus NBRC 13017^T, Streptomyces umbrinus JCM 4521^T and other species with validly published names in the genus Streptomyces. However, the digital DNA-DNA relatedness and average nucleotide identity values between ZYC-3^T, BH-SS-21^T, and their closely related strains were significantly lower than the recommended threshold values. Also, phenotypic, chemotaxonomic and genetic features distinguished ZYC-3^T and BH-SS-21^T from their reference strains. On the basis of their genotypic and phenotypic characteristics, strains ZYC-3^T and BH-SS-21^T were classified as representing novel species of the genus Streptomyces under the names Streptomyces liliifuscus sp. nov. ZYC-3^T (=CICC 25040^T=JCM 34560^T=MCCC 1K04978^T) and Streptomyces liliiviolaceus sp. nov. BH-SS-21^T (=MCCC 1K06236^T=JCM 34767^T), respectively.

Keywords: Actinobacteria; Streptomyces liliifuscus sp. nov; Streptomyces liliiviolaceus sp. nov.; comparative genomic analysis; ginger plague; polyphasic taxonomy.