Phomopsis longanae Chi causing the pulp breakdown of fresh longan fruit through affecting reactive oxygen species metabolism

Jing Yu; Yazhen Chen; Hetong Lin; Yifen Lin; Mengshi Lin; Yi Zheng; Zhongqi Fan

doi:10.1016/j.fochx.2022.100301

Phomopsis longanae Chi causing the pulp breakdown of fresh longan fruit through affecting reactive oxygen species metabolism

Food Chem X. 2022 Apr 6:14:100301. doi: 10.1016/j.fochx.2022.100301. eCollection 2022 Jun 30.

Authors

Jing Yu^{1

2}, Yazhen Chen^{1

2}, Hetong Lin^{1

2}, Yifen Lin^{1

2}, Mengshi Lin³, Yi Zheng^{1

2}, Zhongqi Fan^{1

2}

Affiliations

¹ Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
² Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products, Fujian Province University, Fuzhou, Fujian 350002, China.
³ Food Science Program, Division of Food, Nutrition & Exercise Sciences, University of Missouri, Columbia, MO 65211-5160, USA.

Abstract

Phomopsis longanae Chi is a crucial pathogen causing fruit spoilage in postharvest fresh longan. The influence of P. longanae invasion with a suspension containing 1 × 10⁴ P. longanae spores per mL on the breakdown occurrence and ROS metabolism in pulp of longan cv. Fuyan during storage at 28 °C was explicated. Compared to control group, more severe development of pulp breakdown (PB), higher PB index, O₂ ^-. generation rate, H₂O₂ and MDA content, but lower SOD, APX and CAT activities, GSH, AsA, flavonoid and total phenolics amounts, ability of scavenging DPPH radical, and reducing power were displayed in the pulp of P. longanae-infected fruit during days 0-5. In this context, P. longanae induced breakdown of longan pulp by reducing the scavenging ability of ROS and increasing the cumulation of ROS, thereby enhancing the structural collapse and lipid peroxidation of cell membrane, which were responsible for the PB of harvested longans.

Keywords: APX, ascorbate peroxidase; AsA, ascorbic acid; CAT, catalase; DPPH, 1,1-diphenyl-2-picrylhydrazyl; Fruit spoilage; GSH, glutathione; H2O2, hydrogen peroxide; Longan; MDA, malondialdehyde; O2–., superoxide anion; P. longanae, Phomopsis longanae Chi; PB, pulp breakdown; Phomopsis longanae Chi; Pulp breakdown; ROS metabolism; ROS scavenging enzyme; ROS, reactive oxygen species; SOD, superoxide dismutase.