Effects of sulfidation of silver nanoparticles on the Ag uptake kinetics in Brassica rapa plants

Abstract

Land application of sewage sludge containing increasing levels of silver nanoparticles (AgNPs) raises concerns about the risk for plant exposure. This study compared the uptake kinetics and distribution of Ag in Brassica rapa seedlings grown in Lufa 2.2 natural soil spiked with 20 nm Ag₂S NPs, with those from 3 to 8 nm AgNPs, 50 nm AgNPs and AgNO₃ exposures (10 mg Ag/kg dry soil). A two-compartment model was used to describe the uptake kinetics of Ag in plants, distinguishing two stages: stage I with increasing Ag uptake followed by stage II with decreasing Ag uptake. The concentration of Ag in roots from Ag₂S NPs was about 14 and 10 times lower than for the other AgNPs and AgNO₃ exposures, respectively, at the end of stage I, with root translocation rate constants being higher for Ag₂S NPs. In stage II, Ag uptake occurred only for the 50 nm AgNPs. The distribution of Ag in B. rapa exposed to pristine, ionic and sulfidized AgNPs differed at the end of exposure. This study shows that Ag uptake and distribution in plants depends on the Ag form in soil, highlighting the importance of studying the environmentally relevant chemical species in NPs risk assessment.

Keywords: Ag dissolution kinetics; Ag distribution in plant; Brassica rapa; Silver nanoparticles; Two-stage two-compartment model.