Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography

Kaloyan Takov; I-Jou Teng; Manuel Mayr

doi:10.1007/978-1-0716-2341-1_3

Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography

Methods Mol Biol. 2022:2504:31-40. doi: 10.1007/978-1-0716-2341-1_3.

Authors

Kaloyan Takov¹, I-Jou Teng², Manuel Mayr³

Affiliations

¹ King's College London British Heart Foundation Centre, School of Cardiovascular Medicine and Sciences, London, UK. kaloyan.takov@kcl.ac.uk.
² King's College London British Heart Foundation Centre, School of Cardiovascular Medicine and Sciences, London, UK.
³ King's College London British Heart Foundation Centre, School of Cardiovascular Medicine and Sciences, London, UK. manuel.mayr@kcl.ac.uk.

PMID: 35467277
DOI: 10.1007/978-1-0716-2341-1_3

Abstract

Circulating extracellular vesicles (EVs) are gaining increased attention as carriers of proteins, nucleic acids, and lipids. Blood contains EVs from different cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a single-step, high-performance size-exclusion chromatography procedure for separation of EVs from most lipoproteins and non-EV proteins, and compare it to ultracentrifugation, still the most commonly used method for EV isolation.

Keywords: Exosomes; Extracellular vesicles; HPLC; Lipoproteins; Plasma; Proteomics; Size-exclusion chromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Gel
Extracellular Vesicles* / metabolism
Lipoproteins / metabolism
Nucleic Acids* / metabolism
Ultracentrifugation

Substances

Lipoproteins
Nucleic Acids

Abstract

Publication types

MeSH terms

Substances

Grants and funding