Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor

Lizhou Xu; Khuloud T Al-Jamal

doi:10.1007/978-1-0716-2341-1_2

Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor

Methods Mol Biol. 2022:2504:21-30. doi: 10.1007/978-1-0716-2341-1_2.

Authors

Lizhou Xu^{1

2}, Khuloud T Al-Jamal³

Affiliations

¹ Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, China.
² Institute for Pharmaceutical Science, King's College London, London, UK.
³ Institute for Pharmaceutical Science, King's College London, London, UK. khuloud.al-jamal@kcl.ac.uk.

PMID: 35467276
DOI: 10.1007/978-1-0716-2341-1_2

Abstract

Exosomes are a type of extracellular vesicles that contain constituents including proteins, DNAs, and RNAs of the cells that secrete them. Cancerous exosomes are potential biomarkers for cancer diagnosis. Biosensors are useful analytical tools for quantification of biomarkers and targeted molecules. An aptasensor uses the aptamer as the biorecognition element to bind to the target and is one main type of biosensors that is promising for exosomes analysis and clinical cancer detection. The assay described in this chapter allows for reliable, sensitive, and specific detection of cancer-derived exosomes using a colorimetric aptasensor that is promising for point-of-care testing.

Keywords: Aptamer; Aptasensor; Biomarker; Biosensor; Cancer detection; Exosomes.

MeSH terms

Aptamers, Nucleotide* / metabolism
Biosensing Techniques*
Colorimetry
Exosomes* / metabolism
Humans
Neoplasms* / diagnosis
Neoplasms* / metabolism

Substances

Aptamers, Nucleotide