A Flexible Electrochemiluminescence Sensor Equipped With Vertically Ordered Mesoporous Silica Nanochannel Film for Sensitive Detection of Clindamycin

Xinjie Wei; Xuan Luo; Shuai Xu; Fengna Xi; Tingting Zhao

doi:10.3389/fchem.2022.872582

A Flexible Electrochemiluminescence Sensor Equipped With Vertically Ordered Mesoporous Silica Nanochannel Film for Sensitive Detection of Clindamycin

Front Chem. 2022 Apr 6:10:872582. doi: 10.3389/fchem.2022.872582. eCollection 2022.

Authors

Xinjie Wei¹, Xuan Luo², Shuai Xu², Fengna Xi², Tingting Zhao¹

Affiliations

¹ Guangxi Medical University Cancer Hospital, Guangxi Medical University, Nanning, China.
² Department of Chemistry, Key Laboratory of Surface and Interface Science of Polymer Materials of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, China.

Abstract

Fast, convenient, and highly sensitive detection of antibiotic is essential to avoid its overuse and the possible harm. Owing to enrichment effect and antifouling ability of ultrasmall nanochannels, the vertically ordered mesoporous silica nanochannel film (VMSF) has great potential in the development of the facile electrochemiluminescence (ECL) sensor for direct and sensitive analysis of antibiotics in complex samples. In this study, we demonstrated a flexible ECL sensor based on a cost-effective electrode covered with a VMSF for sensitive detection of clindamycin. Polyethylene terephthalate coated with indium tin oxide (PET-ITO) is applied as a flexible electrode to grow VMSF using the electrochemically assisted self-assembly (EASA) method. The negatively charged VMSF nanochannels exhibit significant enrichment toward the commonly used cationic ECL luminophores, tris(2,2-bipyridyl) dichlororuthenium (II) (Ru (bpy)₃ ²⁺). Using the enhanced ECL of Ru (bpy)₃ ²⁺ by clindamycin, the developed VMSF/PET-ITO sensor can sensitively detect clindamycin. The responses were linear in the concentration range of 10 nM-25 μM and in the concentration range of 25-70 μM. Owing to the nanoscale thickness of the VMSF and the high coupling stability with the electrode substrate, the developed flexible VMSF/PET-ITO sensor exhibits high signal stability during the continuous bending process. Considering high antifouling characteristic of the VMSF, direct analysis of clindamycin in a real biological sample, human serum, is realized.

Keywords: clindamycin; electrochemiluminescence sensor; flexible electrode; sensitive detection; vertically ordered mesoporous silica nanochannel film.