Ultrasensitive Detection of Ochratoxin A With a Zeolite Imidazolate Frameworks Composite-Based Electrochemical Aptasensor

Xiao Ni; Yuyan Zhang; Chuhan Xue; Xiaojun Chen

doi:10.3389/fchem.2022.858107

Ultrasensitive Detection of Ochratoxin A With a Zeolite Imidazolate Frameworks Composite-Based Electrochemical Aptasensor

Front Chem. 2022 Apr 6:10:858107. doi: 10.3389/fchem.2022.858107. eCollection 2022.

Authors

Xiao Ni¹, Yuyan Zhang¹, Chuhan Xue², Xiaojun Chen^{1

3}

Affiliations

¹ College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, China.
² Shanghai Pudong New District Jincai High School, Shanghai, China.
³ Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China.

Abstract

Ochratoxin A (OTA) is a harmful mycotoxin, which is mainly secreted by Penicillium and Aspergillus species. In this work, an electrochemical aptasensor is presented for OTA detection based on Au nanoparticles (AuNPs) modified zeolite imidazolate frameworks (ZIFs) ZIF-8 platform and duplex-specific nuclease (DSN) triggered hybridization chain reaction (HCR) signal amplification. G-quadruplex-hemin assembled HCR nanowire acted as a nicotinamide adenine dinucleotide (NADH) oxidase and an HRP-mimicking DNAzyme. Besides, thionine (Thi) was enriched as a redox probe for signal amplification in this pseudobienzyme electrocatalytic system. Under the optimal conditions, the analytical response ranged from 1 to 10⁷ fg ml^-1 with a detection limit of 0.247 fg ml^-1. Furthermore, the aptasensor was proven to be applied in real wheat samples with a recovery between 96.8 and 104.2%, illustrating the potential prospects in practical detection.

Keywords: electrochemical aptasensors; mycotoxin; ochratoxin A (OTA); signal amplification strategy; zeolite imidazolate frameworks (ZIF-8).