A Comparison of Photoprotective Mechanism in Different Light-Demanding Plants Under Dynamic Light Conditions

Sheng-Pu Shuang; Jin-Yan Zhang; Zhu Cun; Hong-Min Wu; Jie Hong; Jun-Wen Chen

doi:10.3389/fpls.2022.819843

A Comparison of Photoprotective Mechanism in Different Light-Demanding Plants Under Dynamic Light Conditions

Front Plant Sci. 2022 Apr 6:13:819843. doi: 10.3389/fpls.2022.819843. eCollection 2022.

Authors

Sheng-Pu Shuang^{1

2

3}, Jin-Yan Zhang^{1

2

3}, Zhu Cun^{1

2

3}, Hong-Min Wu^{1

2

3}, Jie Hong^{1

2

3}, Jun-Wen Chen^{1

2

3}

Affiliations

¹ College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
² Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, China.
³ National and Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, China.

Abstract

Light intensity is highly heterogeneous in nature, and plants have evolved a series of strategies to acclimate to dynamic light due to their immobile lifestyles. However, it is still unknown whether there are differences in photoprotective mechanisms among different light-demanding plants in response to dynamic light, and thus the role of non-photochemical quenching (NPQ), electron transport, and light energy allocation of photosystems in photoprotection needs to be further understood in different light-demanding plants. The activities of photosystem II (PSII) and photosystem I (PSI) in shade-tolerant species Panax notoginseng, intermediate species Polygonatum kingianum, and sun-demanding species Erigeron breviscapus were comparatively measured to elucidate photoprotection mechanisms in different light-demanding plants under dynamic light. The results showed that the NPQ and PSII maximum efficiency (F _v'/F _m') of E. breviscapus were higher than the other two species under dynamic high light. Meanwhile, cyclic electron flow (CEF) of sun plants is larger under transient high light conditions since the slope of post-illumination, P700 dark reduction rate, and plastoquinone (PQ) pool were greater. NPQ was more active and CEF was initiated more readily in shade plants than the two other species under transient light. Moreover, sun plants processed higher quantum yield of PSII photochemistry (Φ_PSII), quantum yield of photochemical energy conversion [Y(I)], and quantum yield of non-photochemical energy dissipation due to acceptor side limitation (Y(NA), while the constitutive thermal dissipation and fluorescence (Φ_f,d) and quantum yield of non-photochemical energy dissipation due to donor side limitation [Y(ND)] of PSI were higher in shade plants. These results suggest that sun plants had higher NPQ and CEF for photoprotection under transient high light and mainly allocated light energy through Φ_PSII and Φ_NPQ, while shade plants had a higher Φ_f,d and a larger heat dissipation efficiency of PSI donor. Overall, it has been demonstrated that the photochemical efficiency and photoprotective capacity are greater in sun plants under transient dynamic light, while shade plants are more sensitive to transient dynamic light.

Keywords: cyclic electron flow; dynamic light; light energy distribution; non-photochemical quenching; photoprotection.