Microarray dataset supporting a role for ATF4 in isoginkgetin-induced gene expression in HCT116 cells

Erin van Zyl; Victoria Tolls; Bruce C McKay

doi:10.1016/j.dib.2022.108126

Microarray dataset supporting a role for ATF4 in isoginkgetin-induced gene expression in HCT116 cells

Data Brief. 2022 Apr 1:42:108126. doi: 10.1016/j.dib.2022.108126. eCollection 2022 Jun.

Authors

Erin van Zyl¹, Victoria Tolls¹, Bruce C McKay^{1

2}

Affiliations

¹ Department of Biology, Carleton University.
² Institute of Biochemistry, Carleton University.

Abstract

Isoginkgetin (IGG) is a compound originally derived from the leaves of Ginkgo biloba trees. It was subsequently identified through a chemical screen to be an inhibitor of both the major and minor spliceosome, with an IC50 value of 30 µM [1]. Little is currently known about the overall effects of spliceosome inhibition on human cells. Here, we treated HCT116 and a p53 null subline of colon cancer cells with 30 µM IGG for 8 hours. Total RNA was isolated, and Affymetrix oligonucleotide microarray analysis was completed using samples from two biologically independent experiments. A relatively small number of transcripts were differentially expressed in these cell lines. There was considerable overlap in the upregulated but not the downregulated transcripts. PANTHER Reactome analysis of these shared upregulated transcripts identified enriched pathways involving the ATF4 transcription factor important in the integrated stress response [2].

Keywords: ATF4; Isoginkgetin; Microarray; Spliceosome inhibition.