A regenerable and reducing false-positive fluorescent switch for detection of β-amyloid 1-42 oligomers

Shuang Tan; Shunling Li; Congkui Tang; Xiongfei Bai; Xin Ran; Qing Qu; Lei Li; Long Yang

doi:10.1016/j.talanta.2022.123461

A regenerable and reducing false-positive fluorescent switch for detection of β-amyloid _1-42 oligomers

Talanta. 2022 Aug 15:246:123461. doi: 10.1016/j.talanta.2022.123461. Epub 2022 Apr 13.

Authors

Shuang Tan¹, Shunling Li¹, Congkui Tang¹, Xiongfei Bai¹, Xin Ran², Qing Qu³, Lei Li⁴, Long Yang⁵

Affiliations

¹ School of Chemical Science and Technology, Yunnan University, Kunming, 650091, China.
² College of Chemical Engineering, Southwest Forestry University, Kunming, 650224, China.
³ School of Chemical Science and Technology, Yunnan University, Kunming, 650091, China. Electronic address: quqing@ynu.edu.cn.
⁴ State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, China. Electronic address: leelei@ynu.edu.cn.
⁵ International Joint Research Center for Biomass Materials, Yunnan Province Key Lab of Wood Adhesives and Glued Products, College of Materials Science and Engineering, Southwest Forestry University, Kunming, 650224, China. Electronic address: long133109070@126.com.

PMID: 35461117
DOI: 10.1016/j.talanta.2022.123461

Abstract

The conventional fluorescence analysis methods for disease identification are vulnerable to the restriction with false-positive. Here, a fluorescent switch with high efficiency and regeneration by the black phosphorus (BP) nanosheets-regulated was developed to overcome false-positive issue in the assay of β-amyloid_1-42 oligomers (Aβ) process. The Aβ was rapidly recognized using the fluorescent emitter-nitrogen-doped carbon nanodots (N-CDs) under the regulation of BP nanosheets, while the N-CDs alone cannot recognize Aβ without the introduction of BP. The fluorescence analysis methods exhibited a wide sensing range of 0.25-15.0 ng/mL and a low detection limit of 83 pg/mL for Aβ analysis, which was superior to the reported fluorescence analysis method. Further, BP nanosheets were recycled, demonstrating the fluorescent switch with highly efficient, stable, and regenerable. This provides a new idea for developing high-efficiency and high-precision fluorescence detection platform.

Keywords: BP nanosheets; Fluorescent switch; Reducing false-positive; Regenerable.

MeSH terms

Amyloid beta-Peptides
Carbon
Fluorescent Dyes
Nitrogen
Peptide Fragments
Phosphorus
Quantum Dots*

Substances

Amyloid beta-Peptides
Fluorescent Dyes
Peptide Fragments
amyloid beta-protein (1-42)
Phosphorus
Carbon
Nitrogen