Resistance properties and adaptation mechanism of cadmium in an enriched strain, Cupriavidus nantongensis X1T

Liancheng Fang; Hao Zhu; Yuehan Geng; Genrong Zhang; Houpu Zhang; Taozhong Shi; Xiangwei Wu; Qing X Li; Rimao Hua

doi:10.1016/j.jhazmat.2022.128935

Resistance properties and adaptation mechanism of cadmium in an enriched strain, Cupriavidus nantongensis X1^T

J Hazard Mater. 2022 Jul 15:434:128935. doi: 10.1016/j.jhazmat.2022.128935. Epub 2022 Apr 14.

Authors

Liancheng Fang¹, Hao Zhu², Yuehan Geng², Genrong Zhang², Houpu Zhang¹, Taozhong Shi², Xiangwei Wu¹, Qing X Li³, Rimao Hua⁴

Affiliations

¹ Anhui Provincial Key Laboratory for Quality and Safety of Agri-Products, School of Resource & Environment, Anhui Agricultural University, Hefei, Anhui 230036, China; Institute for Green Development, Anhui Agricultural University, Hefei, Anhui 230036, China.
² Anhui Provincial Key Laboratory for Quality and Safety of Agri-Products, School of Resource & Environment, Anhui Agricultural University, Hefei, Anhui 230036, China.
³ Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East-West Road, Honolulu, HI 96822, United States.
⁴ Anhui Provincial Key Laboratory for Quality and Safety of Agri-Products, School of Resource & Environment, Anhui Agricultural University, Hefei, Anhui 230036, China; Institute for Green Development, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: rimaohua@ahau.edu.cn.

PMID: 35461001
DOI: 10.1016/j.jhazmat.2022.128935

Abstract

Bacterial adaption to heavy metal stress is a complex and comprehensive process of multi-response regulation. However, the mechanism is largely unexplored. In this study, cadmium (Cd) resistance and adaptation mechanism in Cupriavidus nantongensis X1^T were investigated. Strain X1^T could resist the stress of 307 mg/L Cd²⁺ and remove 70% Cd²⁺ in 48 h. Spectroscopic analyses suggested interactions between Cd²⁺ with C-N, -COOH, and -NH ligands of extracellular polymeric substances. Whole-genome sequencing found that the resistance of Cd²⁺ in strain X1^T was caused by the joint action of Czc and Cad systems. Cd²⁺ at 20 mg/L elicited differential expression of 1157 genes in strain X1^T. In addition to the reported effects of uptake, adsorption, effluxion, and accumulation system, the oxidative stress system, Type-VI secretory protein system, Fe-S protein synthesis, and cysteine synthesis system in strain X1^T were involved in the Cd²⁺ resistance and accumulation. The intracellular accumulation content of Cd²⁺ in strain X1^T was higher than the extracellular adsorption content made strain X1^T to be an important resource strain in the bioremediation of Cd-contaminated sewage. The results provide a theoretical network for understanding the complex regulatory system of bacterial resistance and adaptation of Cd against stressful environments.

Keywords: Bioaccumulation; Bioremediation; Cd; Cupriavidus nantongensis X1(T); Resistance.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
Cadmium / metabolism
Cadmium / toxicity
Cupriavidus* / genetics
Cupriavidus* / metabolism
Metals, Heavy* / metabolism

Substances

Metals, Heavy
Cadmium

Supplementary concepts

Cupriavidus nantongensis