Enhanced biological antimony removal from water by combining elemental sulfur autotrophic reduction and disproportionation

Abstract

Antimony (Sb), a toxic metalloid, has serious negative effects on human health and its pollution has become a global environmental problem. Bio-reduction of Sb(V) is an effective Sb-removal approach. This work, for the first time, demonstrates the feasibility of autotrophic Sb(V) bio-reduction and removal coupled to anaerobic oxidation of elemental sulfur (S⁰). In the S⁰-based biological system, Sb(V) was reduced to Sb(III) via autotrophic bacteria by using S⁰ as electron donor. Meanwhile, S⁰ disproportionation reaction occurred under anaerobic condition, generating sulfide and SO₄^2- in the bio-systems. Subsequently, Sb(III) reacted with sulfide and formed Sb(III)-S precipitate, achieving an effective total Sb removal. The precipitate was identified as Sb₂S₃ by SEM-EDS, XPS, XRD and Raman spectrum analyses. In addition, it was found that co-existing nitrate inhibited the Sb removal, as nitrate is the favored electron acceptor over Sb(V). In contrast, the bio-reduction of co-existing SO₄^2- enhanced sulfide generation, followed by promoting Sb(V) reduction and precipitation. Illumina high-throughput sequencing analysis revealed that Metallibacterium, Citrobacter and Thiobacillus might be responsible for Sb(V) reduction and S⁰ disproportionation. This study provides a promising approach for the remediation of Sb(V)-contaminated water.

Keywords: Antimonate; Antimony trisulfide; Sulfur autotrophic reduction; Sulfur disproportionation.