Effects of different reduced sulfur forms as electron donors in the start-up process of short-cut sulfur autotrophic denitrification

Yan Yuan; Xiang Li; Wei Li; Miao Shi; Mao Zhang; Pei-Lin Xu; Bo-Lin Li; Yong Huang

doi:10.1016/j.biortech.2022.127194

Effects of different reduced sulfur forms as electron donors in the start-up process of short-cut sulfur autotrophic denitrification

Bioresour Technol. 2022 Jun:354:127194. doi: 10.1016/j.biortech.2022.127194. Epub 2022 Apr 20.

Authors

Yan Yuan¹, Xiang Li², Wei Li³, Miao Shi³, Mao Zhang³, Pei-Lin Xu³, Bo-Lin Li⁴, Yong Huang¹

Affiliations

¹ School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou 215009, China.
² School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou 215009, China. Electronic address: anammox@126.com.
³ School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China.
⁴ School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan, Hubei 430070, China.

PMID: 35452827
DOI: 10.1016/j.biortech.2022.127194

Abstract

In this study, two short-cut sulfur autotrophic denitrification (SSADN) reactors were initiated using different reduced sulfur forms as electron donors and their effects on the start-up speed of the SSADN process, NO₂^--N accumulation characteristics, and microbial community were investigated. Results revealed that during the same period, due to the relatively slow S⁰ dissolution rate, the NO₂^--N production rate realized by microorganisms in S⁰-SSADN (NO₂^--N production rate (NPR), 174 mg/(L·d)) was significantly slower than S^2--SSADN (NPR, 679 mg/(L·d)). The NO₂^--N accumulation efficiency (NAE) was maintained > 80%, which was significantly higher than S^2--SSADN. In the SSADN system using different reduced sulfur forms, the microbial community structure and abundance considerably differed. The main sulfur-oxidizing bacteria (SOB) in S⁰-SSADN were Sulfurimonas (6.5%) and Thiobacillus (5.3%). The main SOB species in S^2--SSADN was Thiomonas (13.6%). Thermomonas played an important role in the two reactors as an important NO₃^--N denitrifying bacteria species.

Keywords: Community abundance; NO(2)(−)-N accumulation efficiency; NO(2)(−)-N production rate; Reduced sulfur forms; Short-cut sulfur autotrophic denitrification (SSADN); Start-up.

MeSH terms

Autotrophic Processes
Bacteria
Bioreactors*
Denitrification*
Electrons
Nitrates
Nitrogen / chemistry
Nitrogen Dioxide
Sulfur / chemistry

Substances

Nitrates
Sulfur
Nitrogen
Nitrogen Dioxide