GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map

Hirokazu Yagi; Eijiro Amagasa; Masaaki Shiota; Issaku Yamada; Kiyoko F Aoki-Kinoshita; Koichi Kato

doi:10.1093/glycob/cwac025

GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map

Glycobiology. 2022 Jul 13;32(8):646-650. doi: 10.1093/glycob/cwac025.

Authors

Hirokazu Yagi^{1

2}, Eijiro Amagasa³, Masaaki Shiota³, Issaku Yamada⁴, Kiyoko F Aoki-Kinoshita³, Koichi Kato^{1

2

5}

Affiliations

¹ Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
² Exploratory Research Center on Life and Living Systems (ExCELLS), 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787, Japan.
³ Glycan & Life Systems Integration Center (GaLSIC), Soka University, 1-236 Tangi-machi, Hachioji, Tokyo 192-8577, Japan.
⁴ The Noguchi Institute, 1-9-7, Kaga, Itabashi-ku, Tokyo 173-0003, Japan.
⁵ Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787, Japan.

PMID: 35452093
DOI: 10.1093/glycob/cwac025

Abstract

High-performance liquid chromatography (HPLC) elution data provide a useful tool for quantitative glycosylation profiling, discriminating isomeric oligosaccharides. The web application Glycoanalysis by the Three Axes of MS and Chromatography (GALAXY), which is based on the three-dimensional HPLC map of N-linked oligosaccharides with pyridyl-2-amination developed by Dr. Noriko Takahashi, has been extensively used for N-glycosylation profiling at molecular, cellular, and tissue levels. Herein, we describe the updated GALAXY as version 3, which includes new HPLC data including those of glucuronylated and sulfated glycans, an improved graphical user interface using modern technologies, and linked to glycan information in GlyTouCan and the GlyCosmos Portal. This liaison will facilitate glycomic analyses of human and other organisms in conjunction with multiomics data.

Keywords: N-linked oligosaccharide; glycosylation profiling; mass spectrometry; pyridyl-2-amination; three-dimensional HPLC map.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / methods
Glycosylation
Humans
Oligosaccharides* / chemistry
Polysaccharides* / chemistry

Substances

Oligosaccharides
Polysaccharides