Trimethyl chitosan postoperative irrigation solution modulates inflammatory cytokines related to adhesion formation

Abstract

Lavage or irrigation has been instilled in surgical practice for wound clearance and surgical site infection prevention during and after surgery. Herein, we developed a new irrigation solution using trimethyl chitosan (TMC), a quaternized chitosan derivative. The TMC-saline irrigation solution developed in the study possesses highly effective bactericidal properties with hemostatic and anti-adhesion properties. The anti-adhesion property of TMC was investigated in relation to inflammatory cytokine response and wound healing. TMC-saline irrigation solution showed reduced pro-inflammatory cytokine protein and gene expressions relevant in the cascade of wound healing and cytokine-related orchestration of postoperative adhesion formation. Further development of this multifunctional TMC-saline irrigation solution can be beneficial for surgical applications and postoperative wound management.

Keywords: Anti-adhesion; Anti-inflammatory; Chitosan; Irrigation; Trimethyl chitosan.