Expression and single nucleotide polymorphisms of vitamin D receptor gene in cows with puerperal metritis in Egypt

Hala Abdelmenem Rizk Saed; Hussam Mohamed Mohamed Ibrahim; Mahmoud Abbas Elgabry; Sabry Ahmed El-Khodery

doi:10.1016/j.theriogenology.2022.03.019

Expression and single nucleotide polymorphisms of vitamin D receptor gene in cows with puerperal metritis in Egypt

Theriogenology. 2022 Jul 1:186:108-113. doi: 10.1016/j.theriogenology.2022.03.019. Epub 2022 Apr 1.

Authors

Hala Abdelmenem Rizk Saed¹, Hussam Mohamed Mohamed Ibrahim², Mahmoud Abbas Elgabry³, Sabry Ahmed El-Khodery⁴

Affiliations

¹ Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. Electronic address: halasaed14@gmail.com.
² Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. Electronic address: hussam-habosha@mans.edu.eg.
³ Department of Animal Reproduction and Artificial Insemination, National Research Center, Cairo, Egypt. Electronic address: mahmoud1abbas@gmail.com.
⁴ Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. Electronic address: khodery@mans.edu.eg.

PMID: 35447479
DOI: 10.1016/j.theriogenology.2022.03.019

Abstract

The aim of this work is to study the expression profile of the vitamin D receptor (VDR), 1-α hydroxylase enzyme, and chemokine regulated on activation normal T-cell expressed and secreted genes (RANTES) genes in dairy cows with puerperal metritis, as well as to study the association between polymorphisms in the VDR gene and occurrence of such disease condition, which is considered a key to advances in the preventive medicine for such a problem in the future. Blood samples were collected from 60 dairy cows; from which 48 dairy cows proved to suffer puerperal metritis and other 12 apparently healthy recent parturient dairy cows were selected randomly for assessment the fold change variation in the expression profiles of the studied genes. Depending on the clinical examination, the normal cut-off point of the serum calcium, phosphorus, glucose, magnesium, potassium, sodium, and chloride, and the normal reference values of parathyroid hormone (PTH), 18 out of 48 dairy cows suffered puerperal metritis with a normal metabolic profile, which underwent further molecular analysis. In the studied dairy cows that suffered puerperal metritis with a normal metabolic profile, the expression pattern of the VDR and RANTES genes showed a significant up-regulation (P < 0.05), whereas the expression pattern of 1α-hydroxylase enzyme gene showed a significant down-regulation (P < 0.05) in comparison with those of healthy recent parturient dairy cows. The sequenced regions of the VDR gene (exon 7, partial intron 6, partial intron 7) didn't exhibit polymorphisms in their nucleotide sequence, and consecutively their amino acid sequence when compared to the published sequence on the gene bank. The results propose that the VDR gene (exon 7, partial intron 6, partial intron 7) polymorphisms do not play a role in the pathogenesis of puerperal metritis in dairy cows, excluding a role of the vitamin D in puerperal metritis.

Keywords: 1-α hydroxylase enzyme; Dairy cow; Puerperal metritis; RANTES; Vitamin D receptor.

MeSH terms

Animals
Cattle / genetics
Cattle Diseases* / diagnosis
Chemokine CCL5
Egypt / epidemiology
Endometritis* / veterinary
Female
Mixed Function Oxygenases
Polymorphism, Single Nucleotide
Receptors, Calcitriol / genetics

Substances

Chemokine CCL5
Receptors, Calcitriol
Mixed Function Oxygenases