The effect of high humidity hot air impingement blanching on the changes in cell wall polysaccharides and phytochemicals of okra pods

J Sci Food Agric. 2022 Oct;102(13):5965-5973. doi: 10.1002/jsfa.11949. Epub 2022 May 4.

Abstract

Background: Okra pods contain heat-sensitive substances, such as phenolic compounds and other phytochemicals that can be degraded when okra pods are subjected to heat treatment. The understanding of the impact of high humidity hot air impingement blanching (HHAIB) on the changes in physicochemical properties of polysaccharides and phytochemicals of okra pods is of great importance because over-blanching may result in cell membrane disruption and changes in biologically active compounds under prolonged exposure to the thermal treatment. Therefore, the present study aimed to investigate the effect of HHAIB on the changes in physicochemical properties of pectins and phytochemicals extracted from okra pods.

Results: Both the HHAIB time and method of extraction influenced their physicochemical characteristics and biological activity. Pectin fractions subjected to HHAIB were composed of polygalacturonic acid, rhamnogalacturonan, glucomannan, galactan, mannose, arabinose, rhamnose, calcium pectate and arabinogalactan. The contents of total phenolics, total flavonoids and antioxidant activity of extracts mostly increased during HHAIB (i.e. up to 19.0%, 13.2% and 35.3%, respectively). However, HHAIB reduced the chlorophyll-a (up to 55.7%) and lycopene (up to 52.6%) contents of okra pods.

Conclusion: The acquired knowledge may be useful for better understanding and optimization of technologies based on HHAIB treatment. The HHAIB treated okra can be a promising natural alternative in different applications, including its use as a replacement of some ingredients in food or non-food systems as a result of richness in polysaccharides and polyphenols, as well as high antioxidant properties. © 2022 Society of Chemical Industry.

Keywords: Fourier-transform infrared spectroscopy; high-humidity hot air impingement blanching; okra pods; principal component analysis.

MeSH terms

  • Abelmoschus* / chemistry
  • Antioxidants / chemistry
  • Cell Wall / metabolism
  • Hot Temperature
  • Humidity
  • Phytochemicals / analysis
  • Polysaccharides / chemistry

Substances

  • Antioxidants
  • Phytochemicals
  • Polysaccharides