Co-exchanged montmorillonite: a potential antibacterial agent with good antibacterial activity and cytocompatibility

Shun Yang; Yingqi Ji; Fanyan Deng; Xiaojiang Sun; Congqin Ning

doi:10.1039/d2tb00032f

Co-exchanged montmorillonite: a potential antibacterial agent with good antibacterial activity and cytocompatibility

J Mater Chem B. 2022 May 18;10(19):3705-3715. doi: 10.1039/d2tb00032f.

Authors

Shun Yang^{1

2}, Yingqi Ji^{1

2}, Fanyan Deng¹, Xiaojiang Sun³, Congqin Ning^{1

4}

Affiliations

¹ State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
² Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
³ Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. sxj603@126.com.
⁴ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Frontiers Science Center of Biomimetic Catalysis, Shanghai Normal University, Shanghai 200050, China. cqning@mail.sic.ac.cn.

PMID: 35445236
DOI: 10.1039/d2tb00032f

Abstract

As a biocompatible material with rich resources and economic benefits, montmorillonite (MMT) has been widely used in the antibacterial field as a drug carrier and toxin adsorbent. In addition, the distinctive structure of MMT provides a possibility to tune its property in a wide range through ion-exchange. In this study, Co-montmorillonite (CoMMT) was prepared by the ion-exchanging method in a Co(NO₃)₂ solution and its antibacterial activity and cytocompatibility were investigated. The results showed that Co was introduced into MMT successfully and led to an increase in the interlayer spacing of MMT. Also, CoMMT showed a morphology of irregular aggregates consisting of stacked and intertwined lamellae with a uniform cobalt distribution. Besides, CoMMT had better dispersity and higher specific surface area than unmodified MMT. The antibacterial test results showed that CoMMT had good antibacterial activity against S. aureus and E. coli when the CoMMT concentration was higher than 0.2 mg mL^-1 and 0.4 mg mL^-1, respectively. The possible antibacterial mechanism of CoMMT was speculated and verified by a combination of SEM and EDS results. In addition, CoMMT showed no obvious cytotoxicity to MC3TC-E1 at the observed antibacterial concentration. These findings demonstrated that CoMMT with good biocompatibility and antibacterial activity could be used as a novel antibacterial agent for tissue engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents* / chemistry
Anti-Bacterial Agents* / pharmacology
Bentonite* / chemistry
Bentonite* / pharmacology
Biocompatible Materials / pharmacology
Escherichia coli
Staphylococcus aureus

Substances

Anti-Bacterial Agents
Biocompatible Materials
Bentonite