Analysis of the TREC and KREC Levels in the Dried Blood Spots of Healthy Newborns with Different Gestational Ages and Weights

D A Cheremokhin; K Shinwari; S S Deryabina; M A Bolkov; I A Tuzankina; D A Kudlay

doi:10.32607/actanaturae.11501

Analysis of the TREC and KREC Levels in the Dried Blood Spots of Healthy Newborns with Different Gestational Ages and Weights

Acta Naturae. 2022 Jan-Mar;14(1):101-108. doi: 10.32607/actanaturae.11501.

Authors

D A Cheremokhin^{1

2}, K Shinwari³, S S Deryabina^{1

2

3}, M A Bolkov^{1

3}, I A Tuzankina^{1

3}, D A Kudlay^{4

5}

Affiliations

¹ Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, 620049 Russia.
² Medical Center "Healthcare of mother and child", Yekaterinburg, 620041 Russia.
³ Department of Immunochemistry, Institute of Chemical Engineering of the Ural Federal University, Yekaterinburg, 620083 Russia.
⁴ I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119991 Russia.
⁵ National Research Center, Institute of Immunology Federal Medical-Biological Agency of Russia, Moscow, 115522 Russia.

Abstract

Inborn errors of immunity can be detected by evaluating circular DNA (cDNA) fragments of T- and B-cell receptors (TREC and KREC) resulting from the receptor gene rearrangement in T and B cells. Maturation and activation of the fetal immune system is known to proceed gradually according to the gestational age, which highlights the importance of the immune status in premature infants at different gestational ages. In this article, we evaluated TREC and KREC levels in infants of various gestational ages by real-time PCR with taking into account the newborn's weight and sex. The 95% confidence intervals for TREC and KREC levels (expressed in the number of cDNA copies per 105 cells) were established for different gestational groups. The importance of studying immune system development in newborns is informed by the discovered dependence of the level of naive markers on the gestational stage in the early neonatal period.

Keywords: K-deleting recombination excision circles; T-cell receptor excision circles; inborn error of immunity; primary immunodeficiency; reference value; severe combined immunodeficiency.