Structure and activity of marine bacterial communities responding to plastic leachates

Abstract

Plastic in the ocean releases organic compounds that are able to enter the marine dissolved organic carbon pool and be utilized by heterotrophic bacteria. However, no information is known about which groups of bacteria are able to grow and degrade plastic leachates. Here we characterized a marine bacterial community from the NW Mediterranean Sea growing on plastic leachates and quantified its total activity. We used two petro-based plastics, low density polyethylene (LDPE) and polystyrene, and one biodegradable plastic, polylactic acid (PLA), to generate leachates under irradiated (UV-Vis) and non-irradiated conditions. Then we incubated them with a natural bacterial inoculum and determined the single-cell activity and associated taxonomy of the bacterial groups, using a combination of Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization (CARDFISH) and BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT). The community growing in the leachates was mainly composed of Alteromonas (Gammaproteobacteria), followed by Roseobacter (Alphaproteobacteria) and unclassified Gammaproteobacteria. Overall, marine bacteria in the irradiated treatments showed higher total activity compared to the non-irradiated ones, with the community growing on LDPE's leachates presenting the highest values. The biodegradable PLA leachates presented lower activity than those from petro-based plastics but similar bacterial composition, suggesting that it is possible that PLA could last in the ocean as much as petro-based plastics do. The results from this study show the impact of marine plastic debris in the marine microbial community and the marine carbon cycle.

Keywords: BONCAT; CARD-FISH; DOM; Microbial community; Microplastics; Plastic leaching.