A rapid and sensitive fluorescence-based immunochromatographic test (ICT) was successfully developed to determine chloramphenicol (CAP) levels in three food products. In this method, lanthanide fluorescent microspheres were used as a label to detect CAP in food samples within 30 min quantitatively, and the result was displayed on the test strip reader. After optimizing detection conditions, the detection limit (LOD) for the three food products was 0.048-0.073 ng g-1, and the half-maximal inhibitory concentration (IC50) was 0.27 ng mL-1. Six other veterinary drugs were detected using the test strip, and no cross-reactivity was observed, indicating that the specificity of the method was satisfactory. This method was also successfully applied to determine CAP in honey, egg, and fish samples, with recoveries ranging from 78.73% to 121.12%. The results demonstrated that this test strip had high sensitivity and specificity, and could be used for field detection within 30 min.