Rapid FISH results within one hour

Catherine Wren; Elizabeth Tegg

doi:10.1016/j.cancergen.2022.03.005

Rapid FISH results within one hour

Cancer Genet. 2022 Jun:264-265:66-70. doi: 10.1016/j.cancergen.2022.03.005. Epub 2022 Apr 8.

Authors

Catherine Wren¹, Elizabeth Tegg²

Affiliations

¹ Institute of Clinical Pathology and Medical Research, New South Wales Health Pathology, Westmead Hospital, Westmead, NSW 2145, Australia. Electronic address: Catherine.wren1@health.nsw.gov.au.
² Institute of Clinical Pathology and Medical Research, New South Wales Health Pathology, Westmead Hospital, Westmead, NSW 2145, Australia; University of Sydney, Westmead Medical School, Westmead, NSW 2145, Australia.

PMID: 35436678
DOI: 10.1016/j.cancergen.2022.03.005

Abstract

This study evaluated a rapid fluorescence in-situ hybridization (FISH) method with a novel 10 min hybridization time to identify the presence of recurrent gene re-arrangements in patients with Haematological malignancies (HMs). A method comparison experimental approach was used to compare this rapid method to the standard method. Hybridization results using the rapid method were comparable to standard methods in terms of result, signal strength and hybridization quality. This rapid FISH turn around time (TAT) was 1 h and enabled same day reporting of genetic and morphology results for a prompt diagnosis and timely access to targeted therapies.

Keywords: FISH; Haematological malignancies; Intellifish; Leukaemia diagnosis; Rapid test.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Hematologic Neoplasms* / diagnosis
Hematologic Neoplasms* / genetics
Humans
In Situ Hybridization, Fluorescence / methods