Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells

Zhidong Liu; Zhifang Liu; Laihao Li; Junjie Zhang; Qiancheng Zhao; Na Lin; Wenzhu Zhong; Mei Jiang

doi:10.1002/fsn3.2735

Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells

Food Sci Nutr. 2022 Jan 25;10(4):1093-1102. doi: 10.1002/fsn3.2735. eCollection 2022 Apr.

Authors

Zhidong Liu¹, Zhifang Liu¹, Laihao Li², Junjie Zhang³, Qiancheng Zhao⁴, Na Lin¹, Wenzhu Zhong⁵, Mei Jiang¹

Affiliations

¹ East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences Shanghai China.
² South China Sea Fishery Research Institute Chinese Academy of Fishery Sciences Guangzhou China.
³ College of Food Science and Engineering Jiangsu Ocean University Lianyungang China.
⁴ College of Food Science and Engineering Dalian Ocean University Dalian China.
⁵ Fishery Machinery and Instrument Research Institute Chinese Academy of Fishery Sciences Shanghai China.

Abstract

This study aimed to evaluate the immunomodulatory effect of the polysaccharide from Sinonovacula constricta (SCP-1-1) in RAW264.7 cells. SCP-1-1 with a molecular weight of 440.0 kDa consisted of glucose and mannose. The immunomodulatory assay results showed that SCP-1-1 could significantly enhance phagocytic ability, NO production, and some cytokines (TNF-α, IL-6, and IL-1β) secretion of RAW264.7 cell in a dose-dependent manner. Western blot analysis results demonstrated that SCP-1-1 could regulate the expression levels of the key proteins in the signaling pathways of RAW264.7 cell and might associated with NF-κβ and PI3K signaling pathway. These findings could contribute to elucidate the immunomodulatory activities of the polysaccharide from Sinonovacula constricta.

Keywords: Sinonovacula constricta; immunomodulatory activity; macrophage; polysaccharide.