Leaf Intracellular Water Transport Rate Based on Physiological Impedance: A Possible Role of Leaf Internal Retained Water in Photosynthesis and Growth of Tomatoes

Deke Xing; Renlong Mao; Zhenyi Li; Yanyou Wu; Xiaojie Qin; Weiguo Fu

doi:10.3389/fpls.2022.845628

Leaf Intracellular Water Transport Rate Based on Physiological Impedance: A Possible Role of Leaf Internal Retained Water in Photosynthesis and Growth of Tomatoes

Front Plant Sci. 2022 Apr 1:13:845628. doi: 10.3389/fpls.2022.845628. eCollection 2022.

Authors

Deke Xing¹, Renlong Mao¹, Zhenyi Li¹, Yanyou Wu², Xiaojie Qin¹, Weiguo Fu¹

Affiliations

¹ Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, Institute of Agricultural Engineering, Jiangsu University, Zhenjiang, China.
² State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China.

Abstract

Water consumed by photosynthesis and growth rather than transpiration accounts for only 1-3% of the water absorbed by roots. Leaf intracellular water transport rate (LIWTR) based on physiological impedance (Z) provides information on the transport traits of the leaf internal retained water, which helps determine the intracellular water status. Solanum lycopersicum plants were subjected to five different levels of relative soil water content (SWC _R ) (e.g., 100, 90, 80, 70, and 60%) for 3 months. The leaf water potential (Ψ_L), Z, photosynthesis, growth, and water-use efficiency (WUE) were determined. A coupling model between gripping force and physiological impedance was established according to the Nernst equation, and the inherent LIWTR (LIWTR _i ) was determined. The results showed that LIWTR _i together with Ψ _L altered the intracellular water status as water supply changed. When SWC _R was 100, 90, and 80%, stomatal closure reduced the transpiration and decreased the water transport within leaves. Net photosynthetic rate (P _N) was inhibited by the decreased stomatal conductance (g _s ) or Ψ _L , but constant transport of the intracellular water was conducive to plant growth or dry matter accumulation. Remarkably, increased LIWTR _i helped to improve the delivery and WUE of the retained leaf internal water, which maintained P _N and improved the WUE at 70% but could not keep the plant growth and yields at 70 and 60% due to the further decrease of water supply and Ψ _L . The increased transport rate of leaf intracellular water helped plants efficiently use intracellular water and maintain growth or photosynthesis, therefore, adapting to the decreasing water supply. The results demonstrate that the importance of transport of the leaf intracellular water in plant responses to water deficit by using electrophysiological parameters. However, the LIWTR in this research is not directly linked to the regulation of photosynthesis and growth, and the establishment of the direct relationship between leaf internal retained water and photosynthesis and growth needs further research.

Keywords: electrophysiology; growth; photosynthesis; water potential; water-use efficiency.