Salient Findings on Host Range, Resistance Screening, and Molecular Studies on Sterility Mosaic Disease of Pigeonpea Induced by Pigeonpea sterility mosaic viruses (PPSMV-I and PPSMV-II)

B R Sayiprathap; A K Patibanda; V Prasanna Kumari; K Jayalalitha; H K Ramappa; E Rajeswari; L Karthiba; K Saratbabu; Mamta Sharma; H K Sudini

doi:10.3389/fmicb.2022.838047

Salient Findings on Host Range, Resistance Screening, and Molecular Studies on Sterility Mosaic Disease of Pigeonpea Induced by Pigeonpea sterility mosaic viruses (PPSMV-I and PPSMV-II)

Front Microbiol. 2022 Apr 1:13:838047. doi: 10.3389/fmicb.2022.838047. eCollection 2022.

Authors

B R Sayiprathap^{1

2}, A K Patibanda², V Prasanna Kumari², K Jayalalitha³, H K Ramappa⁴, E Rajeswari⁵, L Karthiba⁵, K Saratbabu², Mamta Sharma¹, H K Sudini¹

Affiliations

¹ International Crops Research Institute for the Semi-Arid Tropics, Hyderabad, India.
² Department of Plant Pathology, Acharya N. G. Ranga Agricultural University, Guntur, India.
³ Department of Crop Physiology, Acharya N. G. Ranga Agricultural University, Guntur, India.
⁴ Department of Plant Pathology, University of Agricultural Sciences, Bengaluru, India.
⁵ Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India.

Abstract

Two distinct emaraviruses, Pigeonpea sterility mosaic virus-I (PPSMV-I) and Pigeonpea sterility mosaic virus-II (PPSMV-II) were found to be associated with sterility mosaic disease (SMD) of pigeonpea [Cajanus cajan (L.) Millsp.]. The host range of both these viruses and their vector are narrow, confined to Nicotiana benthamiana identified through mechanical transmission, and to Phaseolus vulgaris cvs. Top Crop, Kintoki, and Bountiful (F: Fabaceae) through mite transmission. A weed host Chrozophora rottleri (F: Euphorbiaceae) was also infected and tested positive for both the viruses in RT-PCR. Among the wild Cajanus species tested, Cajanus platycarpus accessions 15661, 15668, and 15671, and Cajanus scarabaeoides accessions 15683, 15686, and 15922 were infected by both the viruses and mite vector suggesting possible sources of SMD inoculum. Though accession 15666 of C. platycarpus, 15696 of C. scarabaeoides, and 15639 of Cajanus lanceolatus were infected by both the viruses, no mite infestation was observed on them. Phylogenetic analysis of nucleotide sequences of RNA-1 and RNA-2 of PPSMV-I and PPSMV-II isolates in southern India revealed significant divergence especially PPSMV-II, which is closely related to the Fig mosaic virus (FMV) than PPSMV-I. In multilocation testing of pigeonpea genotypes for their broad-based resistance to SMD for two consecutive years, genotypes ICPL-16086 and ICPL-16087 showed resistance reaction (<10% incidence) in all three locations studied. Overall, the present study gives a clear idea about the host range of PPSMV-I and PPSMV-II, their molecular relationship, and sources of resistance. This information is critical for the development of reliable diagnostic tools and improved disease management strategies.

Keywords: Cajanus cajan; PPSMV-I; PPSMV-II; SMD; host-plant resistance (HPR); host-range.