Fucose-containing Abroma augusta mucilage hydrogel as a potential probiotic carrier with prebiotic function

Anupam Roy; Moumita Patra; Shubhajit Sarkhel; Swaraj Sengupta; Sreyajit Saha; Shipra Jha; Gautam Sarkhel; Shanker L Shrivastava

doi:10.1016/j.foodchem.2022.132941

Fucose-containing Abroma augusta mucilage hydrogel as a potential probiotic carrier with prebiotic function

Food Chem. 2022 Sep 1:387:132941. doi: 10.1016/j.foodchem.2022.132941. Epub 2022 Apr 11.

Authors

Anupam Roy¹, Moumita Patra², Shubhajit Sarkhel³, Swaraj Sengupta³, Sreyajit Saha³, Shipra Jha³, Gautam Sarkhel³, Shanker L Shrivastava⁴

Affiliations

¹ Laboratory of Food Chemistry and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, Jharkhand, India. Electronic address: anupamroy@bitmesra.ac.in.
² Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
³ Laboratory of Food Chemistry and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, Jharkhand, India.
⁴ Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.

PMID: 35430541
DOI: 10.1016/j.foodchem.2022.132941

Abstract

The mucilaginous polysaccharide from Abroma augusta stem was examined for its physicochemical, thermal, and functional behavior and explored as a carrier for probiotic bacteria. Composed of glucose, galactose, rhamnose, galactouronic acid and fucose, Abroma augusta mucilage (AAM) exhibited shear thinning behavior (following power law equation) and gel like characteristic (showing higher G' value than G″ value). AAM promoted the growth of probiotic strains with positive prebiotic scores of 0.5 ± 0.06 and 0.51 ± 0.05 for Lactobacillus acidophilus and Lactobacillus casei, respectively. Further, probiotic strains were embedded in the AAM matrix followed by freeze-drying with embedding efficiency of >95%. Viscoelastic properties were retained substantially in the rehydrated probiotic-embedded matrix. AAM could protect probiotic bacteria in simulated gastrointestinal conditions, at elevated (80 °C, 20 min) and at low (4 °C, 4 months) temperatures ensuring higher viabilities of embedded probiotic cells. Our findings established potential carrier capabilities of AAM polysaccharides for probiotic bacteria with thickening and prebiotic activity.

Keywords: Abroma augusta mucilage; Fucose; Hydrogel; Prebiotic; Probiotics carrier.

MeSH terms

Fucose
Hydrogels
Lactobacillus acidophilus
Polysaccharides
Prebiotics*
Probiotics*

Substances

Hydrogels
Polysaccharides
Prebiotics
Fucose