A novel naphthalimide-based fluorescent probe for the colorimetric and ratiometric detection of SO2 derivatives in biological imaging

Linlin Yang; Nan Yang; Pengli Gu; Yihua Zhang; Xianyu Gong; Shichao Zhang; Junwan Li; Liguo Ji; Guangjie He

doi:10.1016/j.bioorg.2022.105801

A novel naphthalimide-based fluorescent probe for the colorimetric and ratiometric detection of SO₂ derivatives in biological imaging

Bioorg Chem. 2022 Jun:123:105801. doi: 10.1016/j.bioorg.2022.105801. Epub 2022 Apr 10.

Authors

Linlin Yang¹, Nan Yang¹, Pengli Gu¹, Yihua Zhang¹, Xianyu Gong¹, Shichao Zhang¹, Junwan Li¹, Liguo Ji², Guangjie He³

Affiliations

¹ Xinxiang Key Laboratory of Forensic Science Evidence, School of Forensic Medicine, Xinxiang Medical University, Jinsui Road No. 601, Xinxiang, 453003, Henan Province, PR China.
² Xinxiang Key Laboratory of Forensic Science Evidence, School of Forensic Medicine, Xinxiang Medical University, Jinsui Road No. 601, Xinxiang, 453003, Henan Province, PR China. Electronic address: liguoji_academy@126.com.
³ Xinxiang Key Laboratory of Forensic Science Evidence, School of Forensic Medicine, Xinxiang Medical University, Jinsui Road No. 601, Xinxiang, 453003, Henan Province, PR China. Electronic address: guangjiehe@163.com.

PMID: 35429713
DOI: 10.1016/j.bioorg.2022.105801

Abstract

SO₂ is a well-known signal molecule and one of reactive sulfur species, which closely participates in many metabolic processes. While unbalanced metabolism of sulfur dioxide can lead to serious complications of various diseases. Therefore, a rapid and accurate monitoring of SO₂ derivatives with high selectivity and sensitivity would be beneficial for their bio-analytic studies. Herein, a novel ratiometric fluorescent probe (NG-TCF) based on ICT mechanism for monitoring SO₂ was developed. The probe underwent a nucleophilic addition of HSO₃^-/SO₃^2- to give rise to a 120 nm blue-shift dual-emission signal changes in enhanced green channel and subdued red channel under a single wavelength excitation. The probe showed fast response rate (within 7 min), good sensitivity (the detection limit is 1.53 µM), and specific response toward HSO₃^-/SO₃^2- over other bio-species, including H₂S and ClO^-. Moreover, the probe can be applied for visual ratio imaging of exogenous and endogenous SO₂ derivatives in living cells.

Keywords: Cell image; Fluorescence probe; ICT; Ratiometric detection; SO(2) derivatives.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry* / methods
Fluorescent Dyes*
HeLa Cells
Humans
Naphthalimides
Sulfur Dioxide

Substances

Fluorescent Dyes
Naphthalimides
Sulfur Dioxide