Induced antigen-binding polyreactivity in human serum IgA

Ekaterina N Gorshkova; Maxime Lecerf; Irina V Astrakhantseva; Ekaterina A Vasilenko; Olga V Starkina; Natalya A Ilyukina; Petya A Dimitrova; Jordan D Dimitrov; Tchavdar L Vassilev

doi:10.1016/j.imbio.2022.152213

Induced antigen-binding polyreactivity in human serum IgA

Immunobiology. 2022 May;227(3):152213. doi: 10.1016/j.imbio.2022.152213. Epub 2022 Apr 6.

Authors

Ekaterina N Gorshkova¹, Maxime Lecerf², Irina V Astrakhantseva³, Ekaterina A Vasilenko⁴, Olga V Starkina⁴, Natalya A Ilyukina⁴, Petya A Dimitrova⁵, Jordan D Dimitrov², Tchavdar L Vassilev⁶

Affiliations

¹ Center of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave, Nizhny Novgorod 603022, Russian Federation. Electronic address: e.n.gorshkova@gmail.com.
² Centre de Recherche des Cordeliers, INSERM, CNRS, Sorbonne Université, Université de Paris, 15 Rue de l'École de Médecine, 75006 Paris, France.
³ Center of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave, Nizhny Novgorod 603022, Russian Federation; Center of Genetics and Life Sciences, Sirius University of Science and Technology, 1 Olympic Ave, Sochi 354349, Russian Federation.
⁴ Center of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave, Nizhny Novgorod 603022, Russian Federation.
⁵ Department of Immunology, Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev str., 1113 Sofia, Bulgaria.
⁶ Center of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave, Nizhny Novgorod 603022, Russian Federation; Department of Immunology, Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev str., 1113 Sofia, Bulgaria. Electronic address: tchavdarv@gmail.com.

PMID: 35429697
DOI: 10.1016/j.imbio.2022.152213

Abstract

Previous studies have shown that polyreactive antibodies play an important role in the frontline defense against the dissemination of pathogens in the pre-immune host. Interestingly, antigen-binding polyreactivity can not only be inherent, but also acquired post-translationally. The ability of individual monoclonal IgG and IgE antibodies to acquire polyreactivity following contact with various agents that destabilize protein structure (urea, low pH) or have a pro-oxidative potential (heme, ferrous ions) has been studied in detail. However, to the best of our knowledge this property of human IgA has previously been described only cursorily. In the present study pooled human serum IgA and two human monoclonal IgA antibodies were exposed to buffers with acidic pH, to free heme or to ferrous ions, and the antigen-binding behavior of the native and modified IgA to viral and bacterial antigens were compared using immunoblot and ELISA. We observed a dose-dependent increase in reactivity to several bacterial extracts and to pure viral antigens. This newly described property of IgA may have therapeutic potential as has already been shown for pooled IgG with induced polyreactivity.

Keywords: Antibody specificity; Human serum IgA; Induced polyreactivity; Monoclonal IgA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal*
Antibody Specificity
Heme
Humans
Immunoglobulin A
Immunoglobulin G*
Ions

Substances

Antibodies, Monoclonal
Immunoglobulin A
Immunoglobulin G
Ions
Heme