A novel aerobic-anoxic baffled reactor is designed for the effective denitrification of real domestic wastewater without an external carbon source. The flow is divided between two inlets at the beginning of each zone to provide a carbon source for the denitrifying bacteria. The effects of operating parameters such as the ratio of chemical oxygen demand to nitrogen (COD/N), flow division ratio, and hydraulic retention time (HRT) on the nitrogen removal were investigated. The optimum values of COD/N and HRT were estimated using response surface methodology (RSM) coupled with a central composite experimental design. The addition of porous biomass support media considerably improved the denitrification and removal of COD. Furthermore, the aerobic-anoxic system showed high stability against sudden HRT and COD/N ratio changes. The microbial analysis showed that Alcaligenes, Achromobacter, and Bordetella were the dominant denitrifying bacteria in the anoxic zone, whereas other species coexisted in the aerobic zone.
Keywords: Anoxic; Baffled reactor; Denitrification; Nitrogen; Wastewater.
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