Connecting Ras and CTP synthase in Drosophila

Youfang Zhou; Jingnan Liu; Ji-Long Liu

doi:10.1016/j.yexcr.2022.113155

Connecting Ras and CTP synthase in Drosophila

Exp Cell Res. 2022 Jul 1;416(1):113155. doi: 10.1016/j.yexcr.2022.113155. Epub 2022 Apr 12.

Authors

Youfang Zhou¹, Jingnan Liu¹, Ji-Long Liu²

Affiliations

¹ School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
² School of Life Science and Technology, ShanghaiTech University, Shanghai, China. Electronic address: liujl3@shanghaitech.edu.cn.

PMID: 35427600
DOI: 10.1016/j.yexcr.2022.113155

Abstract

CTP synthase (CTPS), the enzyme responsible for the last step of de novo synthesis of CTP, forms filamentous structures termed cytoophidia in all three domains of life. Here we report that oncogenic Ras regulates cytoophidium formation in Drosophila intestines. Overexpressing active Ras induces elongate and abundant cytoophidia in intestinal stem cells (ISCs) and enteroblasts (EBs). Knocking-down CTPS in ISCs/EBs suppresses the over- proliferation phenotype induced by ectopic expression of active Ras. Moreover, disrupting cytoophidium formation increases the number of proliferating cells in the background of overexpressing active Ras. Therefore, our results demonstrate a link between Ras and CTPS.

Keywords: CTP synthase; Cytoophidium; Drosophila; Intestine stem cell; Ras.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbon-Nitrogen Ligases* / genetics
Drosophila*
Intestines
Stem Cells

Substances

Carbon-Nitrogen Ligases
CTP synthetase