This protocol describes the different methods to collect and preserve bark and ambrosia beetles, detailing collecting tools, recording relevant data, and optimizing step-by-step methods to extract beetles from twigs, branches, bark, and trunks. It elaborates on trapping techniques, tools, lures, baits, and beetle preservation. The main rule of manual collecting is to not attempt to pry the insect out of the wood or bark, but instead, remove the wood/bark away from the beetle: gently and systematically. The main rule of trapping is that there is no general attractant; instead, attractants and traps should reflect the ecology of the targeted beetle taxa.