Objective: To review the research progress of different cell seeding densities and cell ratios in cartilage tissue engineering.
Methods: The literature about tissue engineered cartilage constructed with three-dimensional scaffold was extensively reviewed, and the seeding densities and ratios of most commonly used seed cells were summarized.
Results: Articular chondrocytes (ACHs) and bone marrow mesenchymal stem cells (BMSCs) are the most commonly used seed cells, and they can induce hyaline cartilage formation in vitro and in vivo. Cell seeding density and cell ratio both play important roles in cartilage formation. Tissue engineered cartilage with good quality can be produced when the cell seeding density of ACHs or BMSCs reaches or exceeds that in normal articular cartilage. Under the same culture conditions, the ability of pure BMSCs to build hyaline cartilage is weeker than that of pure ACHs or co-culture of both.
Conclusion: Due to the effect of scaffold materials, growth factors, and cell passages, optimal cell seeding density and cell ratio need further study.
目的: 综述软骨组织工程中种子细胞接种密度和比例的研究进展。.
方法: 广泛查阅三维结构中构建组织工程软骨的相关文献，对最常用的种子细胞接种密度与比例进行综述。.
结果: 关节软骨细胞（articular chondrocytes，ACHs）和BMSCs是软骨组织工程中最常用的种子细胞，体内、外实验显示两种细胞均具有一定形成透明软骨的能力。而种子细胞的接种密度和比例对最终形成的组织工程软骨质量有重要影响。ACHs或BMSCs细胞接种密度接近或高于正常关节软骨中的细胞密度时，可构建出质量较好的组织工程软骨。在相同培养条件下，BMSCs在单独应用时形成透明软骨的能力不如ACHs或两者共培养。.
结论: 受支架材料、生长因子、细胞代次等因素影响，软骨组织工程中最优的细胞接种密度与比例仍需进一步研究。.
Keywords: Cartilage tissue engineering; articular chondrocytes; bone marrow mesenchymal stem cells; cell ratio; co-culture; seeding density.