Open-tubular capillary electrochromatography with hydroxypropyl-β-cyclodextrin imprinted polymers: hybrid polyhedral oligomeric silsesquioxane as a coating for enantioseparation

Jian Zhang; Lingling Liang; Yanqing Miao; Yang Yang; Xin Bao; Chunye Liu

doi:10.1039/d2ra00079b

Open-tubular capillary electrochromatography with hydroxypropyl-β-cyclodextrin imprinted polymers: hybrid polyhedral oligomeric silsesquioxane as a coating for enantioseparation

RSC Adv. 2022 Mar 28;12(16):9637-9644. doi: 10.1039/d2ra00079b. eCollection 2022 Mar 25.

Authors

Jian Zhang^{1

2}, Lingling Liang^{1

2}, Yanqing Miao^{1

2}, Yang Yang¹, Xin Bao¹, Chunye Liu^{1

2}

Affiliations

¹ School of Pharmacy, Xi'an Medical University Xi'an 710021 China doris8976@163.com.
² Institute of Medicine, Xi'an Medical University Xi'an 710021 China.

Abstract

A hydroxypropyl-β-cyclodextrin (HP-β-CD) imprinted coating based on polyhedral oligomeric silsesquioxane (POSS) for open tubular electrochromatography was prepared. The mixture of methacryl-POSS (MA0735), HP-β-CD (template), methacrylic acid (MAA, monomer), N,N'-methylenebisacrylamide (MBA, crosslinker) and toluene-dimethyl sulfoxide (porogen) was used to synthesize the chiral selective coating. The influence of synthesis parameters on the imprinting effect and separation performance, including the amount of HP-β-CD, POSS, and MAA, was investigated systemically. The optimum polymerization was prepared by mixing HP-β-CD, MA0735, MAA, and MBA with the molar ratio of 1 : 1.87 : 1.60 : 1.60. Five racemates were separated by the modified capillary columns using aqueous buffer. Column efficiency on the POSS-based MIPs coating column was greater than 22 000 plates/m. MIPs-POSS hybrid coating capillaries had improved resolution (3.36 times) and the greatest resolution was up to 6.15 within 10 min.