A DNA small molecular probe with increasing K+ concentration promoted selectivity

Ya-Ping Gong; Jian Yang; Ji-Wang Fang; Qian Li; Zhi-Yong Yu; Aijiao Guan; Han-Yuan Gong

doi:10.1039/d0ra06274j

A DNA small molecular probe with increasing K⁺ concentration promoted selectivity

RSC Adv. 2021 Apr 22;11(25):15030-15035. doi: 10.1039/d0ra06274j. eCollection 2021 Apr 21.

Authors

Ya-Ping Gong^{1

2}, Jian Yang³, Ji-Wang Fang^{1

3}, Qian Li², Zhi-Yong Yu¹, Aijiao Guan², Han-Yuan Gong³

Affiliations

¹ Department of Chemistry, Renmin University of China Beijing 100872 P. R. China.
² Institute of Chemistry, Chinese Academy of Sciences Zhongguancunbeiyijie 2 Beijing 100190 P. R. China.
³ College of Chemistry, Beijing Normal University Xinjiekouwaidajie 19 Beijing 100875 P. R. China hanyuangong@bnu.edu.cn.

Abstract

DNA small molecular probe study was considered as a promising approach to achieve DNA related disease diagnosis. Most related reports were performed under specific salinity. Herein, 4-imino-3-(pyridin-2-yl)-4H-quinolizine-1-carbonitrile (IPQC) was generated via a facile procedure with high yield (85%). It is found that IPQC could act as a universal probe for most tested ssDNA, dsDNA and G4 DNA in low [K⁺] concentration (less than 20 mM). However, IPQC showed highly selective G4 DNA binding via UV-vis and fluorescence response in increasing [K⁺] (e.g., 150 mM) conditions. The ion atmosphere effects are instructive for DNA probe exploration. This provides guidance for the design, selection and optimization of the probes for target DNA sensing.