New pentacyclic triterpenoids isolated from Leptopus chinensis and their hepatoprotective activities on tert-butyl hydroperoxide-induced oxidative injury

Liping Long; Ye Yang; Tianliang Zhu; Xinxin Zhang; Shizhou Qi; Ting Liu; Kairu Song; Da Wang; Huiyuan Gao

doi:10.1039/d1ra00962a

New pentacyclic triterpenoids isolated from Leptopus chinensis and their hepatoprotective activities on tert-butyl hydroperoxide-induced oxidative injury

RSC Adv. 2021 Apr 1;11(21):12784-12793. doi: 10.1039/d1ra00962a. eCollection 2021 Mar 29.

Authors

Liping Long^{1

2}, Ye Yang^{1

2}, Tianliang Zhu^{1

2}, Xinxin Zhang^{1

2}, Shizhou Qi^{1

2}, Ting Liu^{1

2}, Kairu Song^{1

2}, Da Wang³, Huiyuan Gao^{1

2}

Affiliations

¹ School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University Shenyang 110016 People's Republic of China sypugaohy@163.com.
² Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University Shenyang 110016 People's Republic of China.
³ School of Pharmacy, Shenyang Pharmaceutical University Shenyang 110016 People's Republic of China.

Abstract

Eight unknown pentacyclic triterpenoids (1-4 and 8-11), along with eight known analogues (5-7 and 12-16) have been first isolated from the dried whole plant of Leptopus chinensis. The structures of the new compounds were determined by comprehensive spectroscopic methods, including 1D and 2D NMR, as well as HRESIMS measurements. Meanwhile, the hepatoprotective activities of the isolated compounds were preliminarily evaluated, and the results indicated that compounds 2, 5 and 16 possess potent protective effects on tert-butyl hydroperoxide (t-BHP)-induced oxidative injury in vitro, and further study revealed that 16 significantly alleviates t-BHP-induced hepatotoxicity by effectively improving cell viability and decreasing reactive oxygen species (ROS) generation and the cell apoptosis rate in HepG2 cells.